Effect of LASSBio-1586 (10, 20 and 40 mg/kg, p.o.) and indomethacin (20 mg/kg, p.o.) on cotton pellet-induced granuloma.

<p>Values are expressed as the mean ± S.E.M. (n = 6, per group), where <i>a</i> indicates <i>p</i><0.05, significantly different from the control group, according to ANOVA, followed by Tukey’s test.</p

Results of the docking procedures for LASSBio-1586.

<p><b>(A)</b> Conformations obtained from the most populous and stable cluster in the binding site of COX-2; <b>(B)</b> most stable docked conformation for LASSBio-1586 (Binding Energy = −7.84 Kcal/mol); <b>(C)</b> native conformation of meloxicam (magenta) and most stable conformation for LASSBio-1586 (cyan).</p

Effect of LASSBio-1586 (10, 20 and 40 mg/kg, p.o.), morphine (10 mg/kg, i.p.) and indomethacin (20 mg/kg, i.p.) in the acetic-acid-writhing-induced nociception test in mice (n = 6, per group).

<p>Values are expressed as the mean ± SEM, where <i>a</i> indicates <i>p</i><0.05, significantly different from the control group, according to ANOVA, followed by Tukey’s test.</p

2D comparative representation of the NSAID meloxicam and LASSBio-1586.

<p>2D comparative representation of the NSAID meloxicam and LASSBio-1586.</p

Effect of LASSBio-1586 (40 mg/kg, p.o.) on paw edema induced by histamine in mice.

<p>The sham group was treated only with saline, whereas the control group received saline and histamine. Values are expressed as the mean ± S.E.M. (n = 6, per group), where <i>a</i> indicates <i>p</i><0.05, significantly different from the control group, according to ANOVA, followed by Tukey’s test.</p

Effect of LASSBio-1586 (10, 20 and 40 mg/kg, p.o.) and dexamethasone (2 mg/kg, i.p.) on leukocyte migration into the peritoneal cavity induced by carrageenan in mice.

<p>Values are expressed as the mean ± S.E.M. (n = 6, per group), where <i>a</i> indicates <i>p</i><0.05, significantly different from the control group, according to ANOVA, followed by Tukey’s test.</p

Effect of LASSBio-1586 (10, 20 and 40 mg/kg, p.o.) and indomethacin (20 mg/kg, i.p.) on paw edema induced by carrageenan in mice.

<p>The sham group was treated only with saline, whereas the control group received saline and carrageenan. Values are expressed as the mean ± S.E.M. (n = 6, per group), where <i>a</i> indicates <i>p</i><0.05, significantly different from the control group, according to ANOVA, followed by Tukey’s test.</p

LASSBio-1586, an <i>N</i>-acylhydrazone derivative, attenuates nociceptive behavior and the inflammatory response in mice - Fig 2

<p><b>Effect of LASSBio-1586 (10, 20 and 40 mg/kg, p.o.), morphine (10 mg/kg, i.p.) and indomethacin (20 mg/kg, i.p.) in the first (A) and second (B) phases of the formalin-induced nociception test in mice (n = 6, per group).</b> Values are expressed as the mean ± SEM, where <i>a</i> indicates <i>p</i><0.05, significantly different from the control group, according to ANOVA, followed by Tukey’s test.</p

LASSBio-1586, an <i>N</i>-acylhydrazone derivative, attenuates nociceptive behavior and the inflammatory response in mice - Fig 3

<p><b>Effect of LASSBio-1586 (40 mg/kg, p.o.), LASSBio-1586 (40 mg/kg, p.o.) + ondansetron (OND 0.5 mg/kg, i.p.) and LASSBio-1586 (40 mg/kg, p.o.) + L-NAME (10 mg/kg, i.p.) in the first (A) and second (B) phases of the formalin-induced nociception test in mice (n = 6, per group).</b> Values are expressed as the mean ± S.E.M., where <i>a</i> indicates <i>p</i><0.05, significantly different from the control group, according to ANOVA, followed by Tukey’s test.</p

Comparative <i>in silico</i> physico-chemical properties and ADMET profile of LASSBio-1586 and the anti-inflammatory drugs indomethacin and meloxicam.

<p>Comparative <i>in silico</i> physico-chemical properties and ADMET profile of LASSBio-1586 and the anti-inflammatory drugs indomethacin and meloxicam.</p